googlebookmarking - Es, all proteins were solubilized in electrophoresis sample buffer (2.3 [wt/vol https://googlebookmarking.com/story.php?id=429399 Es, all proteins were solubilized in electrophoresis sample buffer (2.3 [wt/vol] sodium dodecyl sulfate [SDS], 5 [vol/vol] -mercaptoethanol, 25 [vol/vol] glycerol, and 60 mM Tris pH 6.8), and equal volumes of material were resolved in 12 (vol/vol) polyacrylamide gels by SDS-PAGE (30). Resolved material was then transferred to Immobilon-P (Millipore Corp., Bedford, MA) in Tris-glycine buffer (2 Fri, 04 Sep 2020 14:23:43 UTC en