Derivatives (26). The nucleotide sequences of all of the PCRamplified and cloned fragments were confirmed by DNA sequence analysis. Media and Chemicals--L broth (31) was used for growing cells; ampicillin (50 g/ml) was added when necessary. Chemicals were purchased from Nacalai Tesque (Kyoto, Japan), Wako Pure Chemicals (Osaka, Japan), or Sigma-Aldrich Japan K.K. (Tokyo, Japan). Protein Purificati
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