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Nfection for experiments examining development, as presented in Fig. 7. Microscopy. HeLa monolayers were grown on 12-mm-diameter glass coverslips for immunofluorescence analyses. Monolayers were directly infected with Chlamydia followed by transfection with appropriate plasmid DNAs. Unless otherwise indicated, samples were routinely fixed with methanol at ca. 24 h postinfection for immunofluoresce

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