The vector pEMBLyex4, were introduced into the temperature-sensitive eIF2a (sui2-1, TD304-10B) mutant strain. The indicated transformants were streaked on SC-Gal medium, where eIF2a expression was maintained and the viral protein expression was induced, and incubated at the indicated temperatures. Results shown are representative of 4 independent transformants for each plasmid.Rothenburg et al. BM


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