Express vIF2a or K3L under the control of the galactose regulated GAL-CYC1 promoter. Yeast transformants were streaked on synthetic complete medium containing galactose (SC-Gal) and incubated at different temperatures. At permissive temperatures (27 and 30 ) all transformants grew well (Figure 3). However, when incubated at restrictive temperatures (33 and 36 ), only wild type eIF2a was able to
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