Bjected to SDS-PAGE. The three standard proteins were detected by CBB staining, and DnaK (or DnaK-His), DnaJ-His, and 32 were detected by immunoblotting. Gel images were taken with LAS3000 image analyzer, and the quantitation of protein bands was performed using Multi Gauge software. The fraction numbers of the eluted proteins were as follows: -amylase (200 kDa), Fractions 25 and 26; apo-transferr