Bers were as follows: (DnaK)- 32-(DnaJ2) (184 kDa), Fractions 25 and 26; DnaK- 32 (101 kDa) and (DnaJ2)- 32 (114 kDa), Fractions 27 and 28; and DnaJ2 (82 kDa), Fractions 28 ?0. Because DnaJ was eluted over a wide range from the void volume to Fraction 30 (perhaps due to self-aggregation), the fraction numbers corresponding to a free DnaJ dimer (DnaJ2) were not precisely determined. Other Methods--
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