Sample buffer containing 2 (wt/ vol) SDS and 5 (vol/vol) -mercaptoethanol. Before loading, the samples were boiled for 5 min. Western blot analyses were performed with sheep antibodies against HK (Affinity Biologicals) and its degradation products, as described previously (22). Bradykinin measurement. Purified SK or plasmin was incubated with normal human plasma for 15 min, and the bradykinin co

Sample buffer containing 2 (wt/ vol) SDS and 5 (vol/vol) -mercaptoethanol. Before loading, the samples were boiled for 5 min. Western blot analyses were performed with sheep antibodies against HK (Affinity Biologicals) and its degradation products, as described previously (22). Bradykinin measurement. Purified SK or plasmin was incubated with normal human plasma for 15 min, and the bradykinin co

Sample buffer containing 2 (wt/ vol) SDS and 5 (vol/vol) -mercaptoethanol. Before loading, the samples were boiled for 5 min. Western blot analyses were performed with sheep antibodies against HK (Affinity Biologicals) and its degradation products, as described previously (22). Bradykinin measurement. Purified SK or plasmin was incubated with normal human plasma for 15 min, and the bradykinin co