1
Rboxylterminus. The amplicon was subsequently introduced into the pGEM T-Easy vector (Promega) according to the manufacturer's instructions, and the products were bidirectionally sequenced to confirm their identity. The QuikChange SiteDirected Mutagenesis Kit (Stratagene) protocol was used to perform in vitro site-directed mutagenesis to create the 95?98 kcnj2 construct. The primers used in this p
1
Lymorphism in the native Bat1 as well as being relevant for the creation of Bat1 derivatives with novel specificity (dBats). We hypothesize that nonRVD polymorphisms may have two functionally relevant, non-mutually-exclusive, effects. (i) The formation of unique but functionally equivalent repeat interfaces that stabilize the superhelical structure formed by tandem-arranged repeats (4,5) (superstr