Es, all proteins were solubilized in electrophoresis sample buffer (2.3 [wt/vol] sodium dodecyl sulfate [SDS], 5 [vol/vol] -mercaptoethanol, 25 [vol/vol] glycerol, and 60 mM Tris pH 6.8), and equal volumes of material were resolved in 12 (vol/vol) polyacrylamide gels by SDS-PAGE (30). Resolved material was then transferred to Immobilon-P (Millipore Corp., Bedford, MA) in Tris-glycine buffer (2
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