Ing the unpaired t test (comparison of two groups). All samples were analyzed in triplicates, and all experiments were performed at least three times, if not otherwise declared. The bars in the figures indicate the standard deviations (SD).RESULTSActivation of PK and FXII at the surface of S. pyogenes M49 is dependent on streptokinase and plasminogen. The S. pyogenes M1 strain has previously been

Nfection for experiments examining development, as presented in Fig. 7. Microscopy. HeLa monolayers were grown on 12-mm-diameter glass coverslips for immunofluorescence analyses. Monolayers were directly infected with Chlamydia followed by transfection with appropriate plasmid DNAs. Unless otherwise indicated, samples were routinely fixed with methanol at ca. 24 h postinfection for immunofluoresce