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Lyzed its impact on the modifications of histones bound at the MAGE-A1 promoters. To investigate the changes in the histone signature of MAGE-A1 promoter, it was compared in basal MCF-7 cells (no expression of MAGE-A1, Table 1) to the signature in MCF-7 cells stimulated by 5-aza-CdR with/without TSA or transfected with the expression plasmid encoding for BORIS. For these analyses we used antibodie
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E experiments, we chose, therefore, MCF-7 cells, because they do not express MAGE-A1 mRNA (Table 1). Based on the usually low transfection efficiency we sorted the transfected MCF-7 cells from untransfected cells by FACS and observed a transfection efficiency of about 10 . Subsequently, sodium bisulfite mapping showed a demethylation of the MAGE-A1 promoter of approximately 56 (range from 44 to 6
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On acceptance ?Inclusion in PubMed, CAS, Scopus and Google Scholar ?Research which is freely available for redistributionSubmit your manuscript at www.biomedcentral.com/submitDregan et al. BMC Public Health 2011, 11:86 http://www.biomedcentral.com/1471-2458/11/RESEARCH ARTICLEOpen AccessUtility of electronic patient records in primary care for stroke secondary prevention trialsAlex Dregan1*,
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Ter EJ, Tobin JD, Pearson J, Blackman MR: Longitudinal effects of aging on serum total and free testosterone levels in healthy men: Baltimore Longitudinal Study of Aging. J Clin Endocrinol Metab 2001, 86:724-731. 28. Swerdloff RS, Wang C: Androgen deficiency and aging in men. West J Med 1993, 159:579-585. 29. Li Yong, Qian Zhong-Ji, Ryu Bomi, Lee Sang-Hoon, Kim Moon-Moo, Kim SeKwon: Chemical Compo