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Ation of noncovalent complexes of recombinant human monoclonal antibody and antigen using cation exchange, size exclusion chromatography, and BIAcore. Analyt Biochem. 2001;299(2):119?9. Nesbitt A, Fossati G, Bergin M, Stephens P, Stephens S, Foulkes R, et al. Mechanism of action of certolizumab pegol (CDP870): in vitro comparison with other anti-tumor necrosis factor alpha agents. Inflamm Bowel Di
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Es, all proteins were solubilized in electrophoresis sample buffer (2.3 [wt/vol] sodium dodecyl sulfate [SDS], 5 [vol/vol] -mercaptoethanol, 25 [vol/vol] glycerol, and 60 mM Tris pH 6.8), and equal volumes of material were resolved in 12 (vol/vol) polyacrylamide gels by SDS-PAGE (30). Resolved material was then transferred to Immobilon-P (Millipore Corp., Bedford, MA) in Tris-glycine buffer (2
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N of the mAb were shown to be able to discriminate a thermally degraded sample (data not shown), demonstrating that the utilized assays are sensitive to detect differences in activity, if they did exist. It is well established that IgG1 mAbs are efficient mediators of effector function, and are able to bind to many of the known FccRs. A sensitive comparison of these Fcdependent activities is impor
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5 mg dry weight) are made of mixed cellulose esters of acetates or nitrates containing less than 12.6 nitrogen (Figure 1A). To lessen cross contamination of pump tubing and in line chambers, the aerosol pump was applied strictly for aerosol plus the smoke pump strictly for smoke. Just before each aerosol or smoke trial, pump flow prices were equilibrated to 400 ml/min using an Aalborg GFMFrontier
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5 mg dry weight) are made of mixed cellulose esters of acetates or nitrates containing less than 12.6 nitrogen (Figure 1A). To lessen cross contamination of pump tubing and in line chambers, the aerosol pump was applied strictly for aerosol plus the smoke pump strictly for smoke. Just before each aerosol or smoke trial, pump flow prices were equilibrated to 400 ml/min using an Aalborg GFMFrontier
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Rmer employees and stockholders of Amgen, Inc. Yuh-feng Chen, Gwen Maher, and Teresa L. Born are employees and stockholders of Amgen, Inc. Open Access This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, distribution, and reproduction in any medi