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Has been described for a number of bacterial species (for a review, see reference 7). In the case of S. pyogenes, the secreted streptococcal cysteine protease SpeB was found to cleave HK directly (8). In addition, the surface-bound M protein, one of the classical virulence determinants of GAS, has been demonstrated to bind HK, followed by bradykinin generation (9, 10). Binding and assembly of cont
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Care treatment. J Am Ger Soc. 2008;56(5):816?22. 2. Biston P, Aldecoa C, Devriendt J, Madl C, Chochrad D, Vincent JL, De Backer D. Outcome of elderly patients with circulatory failure. Int Care Med. 2014 Jan;40(1):50?. Grant acknowledgment None.Background: Despite the fact that there is evidence of results of the stewardship programme in conventional hospitalization, results in intensive care unit
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Centrifugation, the pellets were washed three times, centrifuged, and resuspended in 200 l of buffer containing a 1 mM concentration of the substrate. After 60 min, the samples were centrifuged, and the absorbance of the supernatants was measured at 405 nm in an enzyme-linked immunosorbent assay (ELISA) reader. Control values (bacteria incubated in buffer) were used as a blank. No endogenous prote
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N activator streptokinase might contribute to contact system activation by S. pyogenes. Here, we report that the human contact system is activated by the action of streptokinase. The role of secreted and surfacebound streptokinase in this process was investigated by comparing an M49 S. pyogenes wild-type strain with its isogenic ska mutant, which is unable to trigger plasmin activity in human plas
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