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There are actually several forms of grinders some even more usual at that point others. I guess you would call him a hardwood sculptor. You need to find a pleasant balance.
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Ckground (Figure 5B). This does not match expectations based on TALEs where only the cryptic N- but not the cryptic C-terminal repeats are essential for DNA binding (26). By contrast, our results suggest that the cryptic C-terminal Bat1 repeat +1, in contrast to the corresponding cryptic TALE repeat +1, makes an unexpectedly strong contribution to activity and thus should be retained for the creat
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Red DNA bases. RVD composition and target sequence are key parameters determining affinity of TALE NA interactions and these were kept constant in our dBat tests as far as possible. For the repeat switch tests, we exchanged repeats with RVDs paired to the same base in BEBat1 allowing the wildtype target construct to be used in each case. For the RVD switch constructs, where possible we exchanged R
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Lymorphism in the native Bat1 as well as being relevant for the creation of Bat1 derivatives with novel specificity (dBats). We hypothesize that nonRVD polymorphisms may have two functionally relevant, non-mutually-exclusive, effects. (i) The formation of unique but functionally equivalent repeat interfaces that stabilize the superhelical structure formed by tandem-arranged repeats (4,5) (superstr
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Lymorphism in the native Bat1 as well as being relevant for the creation of Bat1 derivatives with novel specificity (dBats). We hypothesize that nonRVD polymorphisms may have two functionally relevant, non-mutually-exclusive, effects. (i) The formation of unique but functionally equivalent repeat interfaces that stabilize the superhelical structure formed by tandem-arranged repeats (4,5) (superstr