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Served between early and late SHG-EM injections comparing the data up to 7 weeks post injection, with the exception of week 5, when the threshold for mechanical allodynia was significantly higher in the group injected early post SCI. *p
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Ats are near identical and repeat order does not change the interface between repeats. Given the numerous nonRVD polymorphisms between Bat1 repeats, deletion or insertion of core repeats will always create novel repeat interfaces and should be experimentally validated before use in downstream applications. We next tested acBat1 derivatives where the 82 residues N-terminal of core repeat 1 (acBat1
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E 6x coverage mammalian genomes were used to build a profile hidden Markov model with the software HMMER [81], which was then used to screen the platypus ab initio peptide predictions available from Ensembl. An inferred ancestral HIN domain sequence from the node that predates the split between HIN-A, -B and -C domains was also used to perform this search. The genomes of chicken (Gallus gallus rel
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Rop in withdrawal threshold (Von Frey test) and increased sensitivity to cold stimuli (acetone test) compared to pre-injection responses (P
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Differentially suggests that RVD scaffold can have a functional impact. However, the natural scaffold does not seem to be the optimal one in every case. Custom dBats can be created to target a novel, user-defined sequence We next tested whether the Bat1 repeat array could be fully customized to target a sequence of interest. Based on the two alternative strategies described above, dBatSOX2 -RVD sw
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R, K3L or vIF2a, demonstrating that K3 and vIF2a had no effect on yeast cell growth (Figure 2A). In contrast, induction of PKR expression was toxic in the vector-transformed yeast, whereas the toxicity was suppressed by co-expression of K3L or vIF2a (Figure 2B). Based on the homology of vIF2a with eIF2a throughout the entire ORF we tested whether suppression of PKR toxicity might be caused by the
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Served between early and late SHG-EM injections comparing the data up to 7 weeks post injection, with the exception of week 5, when the threshold for mechanical allodynia was significantly higher in the group injected early post SCI. *p
1
Served between early and late SHG-EM injections comparing the data up to 7 weeks post injection, with the exception of week 5, when the threshold for mechanical allodynia was significantly higher in the group injected early post SCI. *p