Ic immunoprecipitation and input DNA. H3K9, Lysine 9 of histone H3; H4K8, Lysine 8 of histone H4; H3K4, Lysine 4 of histone H3; H4K20, Lysine 20 of histone H4; ac, acetylated; me, monomethylated; me2, dimethylated; me3, trimethylated. The significant p-values are shown.Schwarzenbach et al. BMC Cancer 2014, 14:796 http://www.biomedcentral.com/1471-2407/14/Page 10 ofTherefore, the predominant occurr
Le lots) and biofunctional parameters. The biofunctional parameters tested include an assessment of both binding and function within both the Fab and Fc portions of the mAb.Acknowledgments The authors thank Abhishek Mathur for scientific and technical contributions to the reported data and Jennifer Liu, Jill Crouse-Zeineddini, and Scott Kuhns for guidance on overall similarity assessment strategy.
The GAS M49 591 chromosomal background was generated previously by insertional inactivation of the ska gene (16). The construction of the epf, emm49, and prtF2 mutant strains from GAS M49 591 has been described previously (17?0). The GAS strains were cultured in Todd-Hewitt broth (THB; Invitrogen) at 37 under a 5 CO2 and 20 O2 atmosphere. Materials. Pooled normal plasma and plasma deficient in
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