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E the reporter to levels above background. If we assume that each repeat contributes a certain amount of affinity to the Bat1?Nucleic Acids Research, 2014, Vol. 42, No. 11BEBat1 interaction then fewer than 17 repeats may simply be insufficient for an interaction strong enough to lead to reporter activation. This is in accordance with results from TALE repeat arrays showing that a certain number of
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Ckground (Figure 5B). This does not match expectations based on TALEs where only the cryptic N- but not the cryptic C-terminal repeats are essential for DNA binding (26). By contrast, our results suggest that the cryptic C-terminal Bat1 repeat +1, in contrast to the corresponding cryptic TALE repeat +1, makes an unexpectedly strong contribution to activity and thus should be retained for the creat
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