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Supernatant from a 10-ml THB overnight culture. The plasminspecific chromogenic substrate was added, and the mixture was incubated at 37 . After 3 h, the absorbance at 405 nm was measured. Instead of the supernatant, 70 l of medium or 30 l of pSK (10 U/ l) served as negative and positive controls, respectively. Electrophoresis and Western blot analysis. Sodium dodecyl sulfatepolyacrylamide gel ele
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N of the mAb were shown to be able to discriminate a thermally degraded sample (data not shown), demonstrating that the utilized assays are sensitive to detect differences in activity, if they did exist. It is well established that IgG1 mAbs are efficient mediators of effector function, and are able to bind to many of the known FccRs. A sensitive comparison of these Fcdependent activities is impor
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Ic immunoprecipitation and input DNA. H3K9, Lysine 9 of histone H3; H4K8, Lysine 8 of histone H4; H3K4, Lysine 4 of histone H3; H4K20, Lysine 20 of histone H4; ac, acetylated; me, monomethylated; me2, dimethylated; me3, trimethylated. The significant p-values are shown.Schwarzenbach et al. BMC Cancer 2014, 14:796 http://www.biomedcentral.com/1471-2407/14/Page 10 ofTherefore, the predominant occurr
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Ic immunoprecipitation and input DNA. H3K9, Lysine 9 of histone H3; H4K8, Lysine 8 of histone H4; H3K4, Lysine 4 of histone H3; H4K20, Lysine 20 of histone H4; ac, acetylated; me, monomethylated; me2, dimethylated; me3, trimethylated. The significant p-values are shown.Schwarzenbach et al. BMC Cancer 2014, 14:796 http://www.biomedcentral.com/1471-2407/14/Page 10 ofTherefore, the predominant occurr
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