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R, K3L or vIF2a, demonstrating that K3 and vIF2a had no effect on yeast cell growth (Figure 2A). In contrast, induction of PKR expression was toxic in the vector-transformed yeast, whereas the toxicity was suppressed by co-expression of K3L or vIF2a (Figure 2B). Based on the homology of vIF2a with eIF2a throughout the entire ORF we tested whether suppression of PKR toxicity might be caused by the
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Introduction:We've all heard that fresh drinking water is going to be much better than oil in the future. There are currently countries with desalination plants and other water recycling systems in place to assist save and produce potable water.None of it are on a scale that can sustain the whole global population forever. The secret to providing water to every home will be
Esia in the plantar test (A) and mechanical allodynia in the Randall-Selitto test (B) in SCI rats after lentiviral injection of GFP (n = 8), EM-1(n = 8), EM-2 (n = 8), SHG (n = 8) and EMs + SHG (n = 10) at two weeks after SCI. Values are expressed as the mean ?S.E.M. *p
Express vIF2a or K3L under the control of the galactose regulated GAL-CYC1 promoter. Yeast transformants were streaked on synthetic complete medium containing galactose (SC-Gal) and incubated at different temperatures. At permissive temperatures (27 and 30 ) all transformants grew well (Figure 3). However, when incubated at restrictive temperatures (33 and 36 ), only wild type eIF2a was able to
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