1
Ated with ammonium sulfate. Proteins that were precipitated in a range of ammonium sulfate concentrations between 0.21 and 0.28 g/ml were dissolved, dialyzed against Buffer A, and loaded onto a HiTrap Q-Sepharose column. Proteins were eluted with a linear gradient of NaCl. The fractions containing DnaJ were dialyzed against Buffer A and applied to a HiTrap heparin column. Proteins were eluted with