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Tion. In control animals receiving the control GFP viral vector injection, GFP fluorescence was apparent inNasirinezhad et al. Molecular Pain 2015, 11:2 http://www.molecularpain.com/content/11/1/Page 6 ofFigure 6 Behavioral outcome of early and late injection of lentiviral constructs. Tactile allodynia in the von Frey test (A), cold allodynia in the acetone test (B), thermal hyperalgesia in the pl

R, K3L or vIF2a, demonstrating that K3 and vIF2a had no effect on yeast cell growth (Figure 2A). In contrast, induction of PKR expression was toxic in the vector-transformed yeast, whereas the toxicity was suppressed by co-expression of K3L or vIF2a (Figure 2B). Based on the homology of vIF2a with eIF2a throughout the entire ORF we tested whether suppression of PKR toxicity might be caused by the

Express vIF2a or K3L under the control of the galactose regulated GAL-CYC1 promoter. Yeast transformants were streaked on synthetic complete medium containing galactose (SC-Gal) and incubated at different temperatures. At permissive temperatures (27 and 30 ) all transformants grew well (Figure 3). However, when incubated at restrictive temperatures (33 and 36 ), only wild type eIF2a was able to

Observations thus far, it appears that neuronal cells are transfected at higher levels than glial cells.Immunodot-blot analysisThe immunodot-blot analysis (Figure 12) indicated the presence of EMs (A) and SHG (B) in cerebrospinal fluid of the animals injected with the viral vectors and the presence of SHG in the spinal cord tissue of treated animals (C). The intensity of the immunoreactive spots s

Ification of hematein as a novel inhibitor of protein kinase CK2 from a natural product libraryMing-Szu Hung1,2,3, Zhidong Xu1, Yu-Ching Lin2,3, Jian-Hua Mao4, ChengTa Yang2,5, Pey-Jium Chang3, David M Jablons*1 and Liang You*Address: 1Thoracic Oncology Laboratory, Department of Surgery, Comprehensive Cancer Center, University of California, San Francisco, CA 94115, USA, 2Division of Pulmonary and