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R, K3L or vIF2a, demonstrating that K3 and vIF2a had no effect on yeast cell growth (Figure 2A). In contrast, induction of PKR expression was toxic in the vector-transformed yeast, whereas the toxicity was suppressed by co-expression of K3L or vIF2a (Figure 2B). Based on the homology of vIF2a with eIF2a throughout the entire ORF we tested whether suppression of PKR toxicity might be caused by the
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S (SHG, EM-1, EM-2) compared to control GFP injection throughout the experiment (overall F(df 4,9) = 37.9; P
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R, K3L or vIF2a, demonstrating that K3 and vIF2a had no effect on yeast cell growth (Figure 2A). In contrast, induction of PKR expression was toxic in the vector-transformed yeast, whereas the toxicity was suppressed by co-expression of K3L or vIF2a (Figure 2B). Based on the homology of vIF2a with eIF2a throughout the entire ORF we tested whether suppression of PKR toxicity might be caused by the
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Social networking marketing for insurance. Remember these benefits and you will have success right away.
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Esia in the plantar test (A) and mechanical allodynia in the Randall-Selitto test (B) in SCI rats after lentiviral injection of GFP (n = 8), EM-1(n = 8), EM-2 (n = 8), SHG (n = 8) and EMs + SHG (n = 10) at two weeks after SCI. Values are expressed as the mean ?S.E.M. *p
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Express vIF2a or K3L under the control of the galactose regulated GAL-CYC1 promoter. Yeast transformants were streaked on synthetic complete medium containing galactose (SC-Gal) and incubated at different temperatures. At permissive temperatures (27 and 30 ) all transformants grew well (Figure 3). However, when incubated at restrictive temperatures (33 and 36 ), only wild type eIF2a was able to
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Site rna: effects of eIF4B and poly(A)-binding protein. J. Biol. Chem. 284: 35461-35470. Khandal, d., Samol, I., Buhr, F., Pollmann, S., Schmidt, H., Clemens, S., Reinbothe, S., and Reinbothe, C. (2009). Singlet oxygen-dependent translational control in the tigrina-d.12 mutant of barley. Proc. Natl. Acad. Sci. U S A 106: 13112-13117. Kim, B.H., Cai, X., vaughn, J.N., and von Arnim, A.G. (2007). On
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