Ted with mortality in elderly (66.42 ?6.47 vs 36.34 ?12.70, P = 0.000) whereas APACHE 11, MV days, Mean blood Glucose (MBG) were not Conclusions: For the similar APACHE 2 score, elderly patients have greater glycemic variability , which could have contributed to the increased mortality. Amongst the glycemic variability indices tested, GLI was found to be better predictor than MBG.References 1. Sub
Ct on the ability of tAg to bind p107 (Fig. 4) or p130 (data not shown). It is likely that both LxCxE motifs contribute to Rb binding, perhaps redundantly, and that to abolish binding activity, double mutants will have to be createdJCV Small t Protein Functionsand tested. It is also possible that neither LxCxE motif is required for Rb binding, as a number of cellular proteins lacking this sequence
Phosphatase activity of PP2A in studies involving a second known binding partner of tAg, the JCV agnoprotein . We were surprised to identify two LxCxE motifs that had been overlooked in the unique region of the JCV tAg; one of these sites is also found in BKV but neither site resides in the corresponding SV40, WUV, KIV or MCV polyomavirus proteins. As predicted, JCV tAg binds members of the re
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The GAS M49 591 chromosomal background was generated previously by insertional inactivation of the ska gene (16). The construction of the epf, emm49, and prtF2 mutant strains from GAS M49 591 has been described previously (17?0). The GAS strains were cultured in Todd-Hewitt broth (THB; Invitrogen) at 37 under a 5 CO2 and 20 O2 atmosphere. Materials. Pooled normal plasma and plasma deficient in
Ults show that changes in the BORIS transcript levels are associated with those of MAGE-A1 and corroborate that BORIS is involved in the activation of MAGE-A1 gene expression.BORIS affects the DNA methylation pattern of MAGE-A1 geneFigure 2 BORIS mRNA expression in MDA-MB-468, MCF-7 and BCM1 cells, untreated or treated with 5-aza-CdR. RT-PCR products of BORIS mRNA were separated on an agarose gel.
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E experiments, we chose, therefore, MCF-7 cells, because they do not express MAGE-A1 mRNA (Table 1). Based on the usually low transfection efficiency we sorted the transfected MCF-7 cells from untransfected cells by FACS and observed a transfection efficiency of about 10 . Subsequently, sodium bisulfite mapping showed a demethylation of the MAGE-A1 promoter of approximately 56 (range from 44 to 6
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